Differences in cytotoxicity between 5% tetracycline hydrochloride and 15% EDTA as root canal irrigant Devi Eka Juniarti, Karlina Samadi, and Achmad Sudirman Department of Conservative Dentistry Faculty of Dentistry, Airlangga University Surabaya - Indonesiaĥ% tetracycline hydrochloride and 15% EDTA as a root canal irrigant have been proven to be able to remove smear layer, open dentinal tubules and have antimicrobial activity.